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Human nucleolar RNA pol I interacting proteins identified by immuno-precipitation and mass spectrometry

ABSTRACT: Ribosome biogenesis is a highly regulated multistep process that controls cell growth and proliferation. The first and key regulatory step of ribosome biogenesis is represented by the transcription of ribosomal RNA (rRNA) genes by RNA polymerase (pol) I in the nucleolus. Activity of RNA pol I is tightly regulated by interactions with many auxiliary factors that mediate promoter recognition and contribute to transcription initiation, elongation and termination. In this study we were able to identify by mass spectrometry some of the known components of the RNA pol I transcribing machinery, and at the same time we identify some novel interactors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Francesca Fabretti

LAB HEAD: Bernhard Schermer

PROVIDER: PXD018143 | Pride | 2020-05-06

REPOSITORIES: Pride

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Che-1/AATF binds to RNA polymerase I machinery and sustains ribosomal RNA gene transcription.

Sorino Cristina C Catena Valeria V Bruno Tiziana T De Nicola Francesca F Scalera Stefano S Bossi Gianluca G Fabretti Francesca F Mano Miguel M De Smaele Enrico E Fanciulli Maurizio M Iezzi Simona S

Nucleic acids research 20200601 11

Originally identified as an RNA polymerase II interactor, Che-1/AATF (Che-1) has now been recognized as a multifunctional protein involved in cell-cycle regulation and cancer progression, as well as apoptosis inhibition and response to stress. This protein displays a peculiar nucleolar localization and it has recently been implicated in pre-rRNA processing and ribosome biogenesis. Here, we report the identification of a novel function of Che-1 in the regulation of ribosomal RNA (rRNA) synthesis, ...[more]

PMID: 32421830

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AATF interactome in human Kms27 cells

Project description:Multiple myeloma is a hematological neoplasm of plasma cells characterized by an abnormal production of immunoglobulins. Che-1/AATF (Che-1) is an RNA binding protein involved in transcription regulation and highly expressed in this malignancy. In order to better characterize its functions in this type of disease, we performed co-immunoprecipitation experiments coupled with a mass-spec analysis by using total MM Kms27 cell lysates. From this analysis we were able to identify more than 600 proteins significantly enriched in Che-1 precipitates. As expected from its role in ribosomal RNA maturation, the Che-1 interactome contains numerous proteins involved in pre-rRNA processing and several ribosomal proteins. Notably, among other proteins interacting with Che-1, we identified many essential components of the paraspeckle which play an important role in the regulation of gene expression.

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2020-01-08 | PXD014469 | Pride

Stably-paused genes revealed through inhibition of transcription initiation by the TFIIH inhibitor Triptolide

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2015-01-05 | E-GEOD-63314 | biostudies-arrayexpress

TAF8 MUTATION CAUSES INTELLECUAL DISABILITY, TFIID DISASSEMBLY WITH NO EFFECT ON RNA POLYMERASE II TRANSCRIPTION

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2018-04-16 | PXD008084 | Pride

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