Mass spectrometry-based analysis of Mycobacterial single-colony proteome

ABSTRACT: Here as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with a hybrid quadrupole mass filter Orbitrap analyser (Q Exactive) and raw data analysed using the MaxQuant Suite and downstream analysis using Perseus software . A total of 7805 unique peptides and 1387 proteins were identified

INSTRUMENT(S):

ORGANISM(S): Mycobacterium Smegmatis (strain Atcc 700084 / Mc(2)155)

SUBMITTER: john Iradukunda  

LAB HEAD: Jonathan Blackburn

PROVIDER: PXD018168 | Pride | 2021-03-11

REPOSITORIES: Pride