Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A quantitative proteomic analysis to reveal effects of N-acetylcystein on H2O2-induced neurotoxicity

ABSTRACT: SK-N-MC cell was used to evaluate effects of N-acetylcystein on H2O2-induced neurotoxicity. Proteomic analysis was conducted in three groups: control, H2O2-treatment (disease group), and NAC pretreatment followed by H2O2-treatment (NAC group).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Van-An Duong

LAB HEAD: Jong-Moon Park

PROVIDER: PXD018177 | Pride | 2020-10-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Effects of N-acetylcysteine on the neurotoxicity induced by acute exposure to acrylamide

Project description:In this study, we used the adult zebrafish model of ACR acute neurotoxicity for determining the suitability of NAC to protect the brain against ACR toxicity. First, the potential protective effects of NAC on the ACR neurotoxicity were evaluated at the behavioral and molecular (transcriptome and proteome) levels. Then, in order to understand the mild to negligible protective effect of NAC, the ability of this drug to cross BBB by passive diffusion was evaluated by different approaches. Whereas the BBB parallel artificial membrane permeability assay (BBB-PAMPA) was used to determine the ability of NAC to cross BBB-like phospholipid membranes by passive diffusion, the determination of the NAC content in the brain provides direct evidences of the BBB permeability in vivo. Finally, we evaluated the ability of drug to recover the depleted GSx stores and to scavenge ACR in the brain of ACR-exposed fish.

2020-05-26 | PXD014228 | Pride

Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes).

2011-07-30 | GSE26961 | GEO

5- AZA treatment of EWS cell lines

Project description:The Ewing Sarcoma cell lines A673, SK-ES-1, and SK-N-MC were treated with without 5-AZA to identify upregulated genes A673, SK-ES-1, and SK-N-MC were treated with 5-AZA

2012-09-01 | E-GEOD-36858 | biostudies-arrayexpress

Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with H2O2 and non-treated cells. A total 6 arrays which includes dye swap were analyzed.

2011-07-29 | E-GEOD-26961 | biostudies-arrayexpress

High-throughout sequencing of ovarian cancer cells treated with TMPyP4 and H2O2

Project description:The high-throughput sequencing technology was performed after the treatment of human ovarian cancer cells A2780 with TMPyP4 and H2O2 purchased from Sigma-Aldrich, to explore the expression of genes related to cell proliferation, DNA damage and ROS levels of ovarian cancer cells A2780 after the treatment of TMPyP4 and H2O2, and to find an interesting target pathway,HIF-1 signaling pathway.

2024-03-25 | GSE262015 | GEO

Human SW480 cells: Untreated vs H2O2 Treated

Project description:ChIP-on-chip tilling array comparing untreated human SW480 cells vs SW480 cells treated with 2mM H2O2 for 30min. Exposing cells to the reactive oxygen species, hydrogen peroxide, recruits DNA methyltransferase 1 (DNMT1) to damaged chromatin. DNMT1 becomes part of a complex(es) containing DNMT3B and members of Polycomb Repressive Complex 4. The goal was to determine the effect of hydrogen peroxide treatment on chromatin, including changes in histone modifications and binding patterns of chromatin-associated proteins. [Agilent-014706]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and EZH2. [Agilent-014707]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and EZH2. [Agilent-026595]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and H3. [Agilent-027811]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Four-mark: H3, 3MeK4H3, AcK16H4, and 3MeK27H3.

2011-12-01 | E-GEOD-32382 | biostudies-arrayexpress

A transcriptome comparison between pao5 vs NIP, ACC-treatment vs Mock (NIP) and H2O2-treatment vs Mock (NIP)

Project description:OsPAO5 mainly functions to oxidize spermine and produce H2O2,which finally affects mesocotyl elongation in rice. Meanwhile ethylene and polyamines synthesis crosstalk has been reported in competing the common substrate. To further confirm the relationship between H2O2 and ethylene in contributing to the mesocotyl length, we conducted a transcriptome comparison between pao5-1 vs NIP, ACC-treatment vs Mock (NIP) and H2O2-treatment vs Mock (NIP).

2023-06-26 | GSE153423 | GEO

Transcriptomic response of ginsenoside Rc (gRc) in C2C12 myotube under H2O2-induced oxidative stress

Project description:Effect of gRc on C2C12 myotube under resting state and H2O2 treatment

2022-03-18 | GSE198653 | GEO

Atf1-mediated active gene response to H2O2 challenge.

Project description:Here we present the study on ChIP-chip mapping of the genomic binding sites for Sty1, Atf1, and the Atf1's binding partner Pcr1; the genome-wide transcriptional profiling of the atf1 and pcr1 strains in response to H2O2; and the phenotypic assessment of ~90 Atf1/Pcr1-bound or unbound genes for growth fitness under H2O2 conditions. ChIP-chip analysis shows that Atf1 and Pcr1 binding sites are overlapped in the genome and constitutively present before H2O2 stress. On the other hand, Sty1 recruitment primarily occurs at the Atf1/Pcr1 binding sites and is induced by H2O2. We found that Atf1/Pcr1 is clearly responsible for the high-level transcriptional response to H2O2. Furthermore, phenotypic assessment indicates that among the H2O2-induced genes, Atf1/Pcr1-bound genes exhibit a higher likelihood of functional requirement for growth fitness under the stress condition than the Atf1/Pcr1-unbound genes do. Notably, we found that the Atf1/Pcr1-bound genes regardless of their responsiveness to H2O2 show a high probability of requirement for growth fitness. . Expression level of genes in triplicates at 0min (without stress) is compared with that at 10, 30, 60, and 120min after stress treatment. ChIP-chip analyses is done for Atf1-HA, pcr1-HA, sty1-HA, Sty1-HA allele in the atf1D or pcr1D background without and with H2O2 (0.5mM for 30min).

2010-07-19 | E-GEOD-13053 | biostudies-arrayexpress

Response of Shewanella oneidensis wild type and arcA mutant strains to H2O2

Project description:We undertook a transcriptional profiling to identify genes coping with H2O2-induced oxidative stress in S. oneidensis. The data revealed that the well conserved scavenging genes such as as ahpCF, katB, and katG formed the first line of defense while iron-sulfur protecting proteins may not play a significant role. Experimental cells collected 5 min after H2O2 treatment; Control cells collected just before H2O2 treatment. In total, 8 samples were studied (4 for wild type and 4 for arcA mutant, 4 replicates).

2012-07-31 | E-GEOD-31053 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data