Proteomics

Dataset Information

0

Glycopeptide Relative Retention Time Prediction


ABSTRACT: A re-analysis of published data-dependent acquisition glycoproteomics datasets to demonstrate relative retention time prediction on glycopeptide identification and disambiguation of adduct states.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Joshua Klein  

LAB HEAD: Joseph Zaia

PROVIDER: PXD018208 | Pride | 2020-04-01

REPOSITORIES: Pride

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Publications

Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.

Klein Joshua J   Zaia Joseph J  

Journal of proteome research 20200410 5


Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties. This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination. To date, approaches to solving these problems have been limited, and few inroads have been made to addres  ...[more]

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