Proteomics

Dataset Information

0

HEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 2 from An et al (2020)


ABSTRACT: HEK293T subject to Torin1 treatment or amino acid withdraw dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMT 10plex labelling was used. Three genotypes, WT (24 fractions), ATG7 KO (HA-9-53-1 single shot), RB1CC1 KO (HA-9-53-2 single shot).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Alban Ordureau  

LAB HEAD: Wade Harper

PROVIDER: PXD018252 | Pride | 2020-04-30

REPOSITORIES: Pride

altmetric image

Publications

Systematic quantitative analysis of ribosome inventory during nutrient stress.

An Heeseon H   Ordureau Alban A   Körner Maria M   Paulo Joao A JA   Harper J Wade JW  

Nature 20200701 7815


Mammalian cells reorganize their proteomes in response to nutrient stress through translational suppression and degradative mechanisms using the proteasome and autophagy systems<sup>1,2</sup>. Ribosomes are central targets of this response, as they are responsible for translation and subject to lysosomal turnover during nutrient stress<sup>3-5</sup>. The abundance of ribosomal (r)-proteins (around 6% of the proteome; 10<sup>7</sup> copies per cell)<sup>6,7</sup> and their high arginine and lysin  ...[more]

Similar Datasets

2020-04-30 | PXD017859 | Pride
2020-07-12 | PXD017852 | Pride
2020-04-30 | PXD017857 | Pride
2020-04-30 | PXD017856 | Pride
2021-04-15 | PXD014260 | Pride
2020-02-12 | PXD015071 | Pride
2020-02-12 | PXD015955 | Pride
2020-04-30 | PXD017858 | Pride
2021-09-09 | PXD022177 | Pride
2022-03-03 | PXD031527 | Pride