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Identify Yeast Proteasome Cross-links with Multiple Ion Fragmentation Methods

ABSTRACT: Use of EThcD, CID, HCD and high energy HCD to identify protein cross-links

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Bingqing Zhao

LAB HEAD: James P Reilly

PROVIDER: PXD018310 | Pride | 2020-06-18

REPOSITORIES: Pride

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Use of Multiple Ion Fragmentation Methods to Identify Protein Cross-Links and Facilitate Comparison of Data Interpretation Algorithms.

Zhao Bingqing B Reilly Colin P CP Davis Caroline C Matouschek Andreas A Reilly James P JP

Journal of proteome research 20200604 7

Multiple ion fragmentation methods involving collision-induced dissociation (CID), higher-energy collisional dissociation (HCD) with regular and very high energy settings, and electron-transfer dissociation with supplementary HCD (EThcD) are implemented to improve the confidence of cross-link identifications. Three different <i>S. cerevisiae</i> proteasome samples cross-linked by diethyl suberthioimidate (DEST) or bis(sulfosuccinimidyl)suberate (BS<sup>3</sup>) are analyzed. Two approaches are i ...[more]

PMID: 32496805

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