Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Pan Fang
LAB HEAD: Kuan-Ting Pan
PROVIDER: PXD018349 | Pride | 2020-08-24
REPOSITORIES: Pride
Fang Pan P Ji Yanlong Y Silbern Ivan I Doebele Carmen C Ninov Momchil M Lenz Christof C Oellerich Thomas T Pan Kuan-Ting KT Urlaub Henning H
Nature communications 20201019 1
Regulation of protein N-glycosylation is essential in human cells. However, large-scale, accurate, and site-specific quantification of glycosylation is still technically challenging. We here introduce SugarQuant, an integrated mass spectrometry-based pipeline comprising protein aggregation capture (PAC)-based sample preparation, multi-notch MS3 acquisition (Glyco-SPS-MS3) and a data-processing tool (GlycoBinder) that enables confident identification and quantification of intact glycopeptides in ...[more]