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Ultrasonic-based filter aided sample preparation as the general method to sample preparation in proteomics

ABSTRACT: In this work, we propose a new high-throughput ultrafast method for large scale proteomics approaches by speeding the classic filter aided sample preparation protocol, FASP. The new US-FASP method matches the analytical minimalism roles as time, cost, sample requirement, reagent consumption, energy requirements and production of waste products are reduced to a minimum while maintaining high sample throughput in a robust manner as all the advantages of the filter aided sample preparation protocol are maintained.

INSTRUMENT(S): impact HD

ORGANISM(S): Escherichia Coli Mus Musculus (mouse)

TISSUE(S): Brain, Liver, Cell Culture

SUBMITTER: Hugo M. Santos

LAB HEAD: Hugo M. Santos

PROVIDER: PXD018360 | Pride | 2020-06-08

REPOSITORIES: Pride

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Ultrasonic-Based Filter Aided Sample Preparation as the General Method to Sample Preparation in Proteomics.

Carvalho Luís B LB Capelo-Martínez José-Luis JL Lodeiro Carlos C Wiśniewski Jacek R JR Santos Hugo M HM

Analytical chemistry 20200611 13

We propose a new high-throughput ultrafast method for large-scale proteomics approaches by speeding up the classic filter aided sample preparation protocol, FASP, from overnight to 2.5 h. Thirty-six samples can be treated in 2.5 h, and the method is scalable to 96-well plate-based pipelines. After a modification of the FASP-tube, the steps of protein reduction, protein alkylation, and protein digestion of complex proteomes are done in just 5.25 min, each one under the effects of an ultrasonic fi ...[more]

PMID: 32484334

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