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MRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1

ABSTRACT: The interactome of Influenza (A/WSN/33) NS1 protein (WT and D92Y mutant) was identifiedy by AP-MS.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Influenza

SUBMITTER: Yushen Du

LAB HEAD: Ren Sun

PROVIDER: PXD018377 | Pride | 2021-09-07

REPOSITORIES: Pride

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mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1.

Du Yushen Y Hultquist Judd F JF Zhou Quan Q Olson Anders A Tseng Yenwen Y Zhang Tian-Hao TH Hong Mengying M Tang Kejun K Chen Liubo L Meng Xiangzhi X McGregor Michael J MJ Dai Lei L Gong Danyang D Martin-Sancho Laura L Chanda Sumit S Li Xinming X Bensenger Steve S Krogan Nevan J NJ Sun Ren R

Nature communications 20200515 1

A comprehensive examination of protein-protein interactions (PPIs) is fundamental for the understanding of cellular machineries. However, limitations in current methodologies often prevent the detection of PPIs with low abundance proteins. To overcome this challenge, we develop a mRNA display with library of even-distribution (md-LED) method that facilitates the detection of low abundance binders with high specificity and sensitivity. As a proof-of-principle, we apply md-LED to IAV NS1 protein. ...[more]

PMID: 32415096

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