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Global Proteomic Profiling in Chronic Iron-Exposed Fallopian Tube Secretory Epithelial Cells

ABSTRACT: We previously published (Oncogenesis, Rockfield et al., 2019) that chronic iron exposure (with ferric ammonium citrate, FAC) in immortalized fallopian tube secretory epithelial cells resulted in increased growth and migratory propensity. Our focused analyses identified a subset of oncogenic markers, including EVI1 (amplified at 3q26.2 in high grade serous epithelial ovarian tumors), that were altered following long term iron treatment. Herein, we have extended these studies to global proteomics analyses via a mass spectrometry (MS)-based approach.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Stanley Stevens

LAB HEAD: Meera Nanjundan

PROVIDER: PXD018416 | Pride | 2021-09-09

REPOSITORIES: Pride

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Global miRNA/proteomic analyses identify miRNAs at 14q32 and 3p21, which contribute to features of chronic iron-exposed fallopian tube epithelial cells.

Chhabra Ravneet R Rockfield Stephanie S Guergues Jennifer J Nadeau Owen W OW Hill Robert R Stevens Stanley M SM Nanjundan Meera M

Scientific reports 20210318 1

Malignant transformation of fallopian tube secretory epithelial cells (FTSECs) is a key contributing event to the development of high-grade serous ovarian carcinoma (HGSOC). Our recent findings implicate oncogenic transformative events in chronic iron-exposed FTSECs, including increased expression of oncogenic mediators, increased telomerase transcripts, and increased growth/migratory potential. Herein, we extend these studies by implementing an integrated transcriptomic and mass spectrometry-ba ...[more]

PMID: 33737539

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