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Identification of barrier-specific proteins in the human brain

ABSTRACT: We present a proteomic analysis of the basal lamina of the human BBB. We combined laser capture microdissection with shotgun proteomics for selective enrichment and identification of specific proteins present in the cerebral microvasculature and arachnoidal vessels collected from normal human brain tissue specimens. Proteins found to be barrier-specific were validated by immunohistochemistry.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Marina Zajec

LAB HEAD: Theo Luider

PROVIDER: PXD018602 | Pride | 2022-01-17

REPOSITORIES: Pride

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Identification of Blood-Brain Barrier-Associated Proteins in the Human Brain.

Zajec Marina M Kros Johan M JM Dekker-Nijholt Diana A T DAT Dekker Lennard J LJ Stingl Christoph C van der Weiden Marcel M van den Bosch Thierry P P TPP Mustafa Dana A M DAM Luider Theo M TM

Journal of proteome research 20201123 1

The blood-brain barrier (BBB) is essential for cerebral homeostasis and controls the selective passage of molecules traveling in and out of the brain. Despite the crucial role of the BBB in a variety of brain diseases and its relevance for the development of drugs, there is little known about its molecular architecture. In particular, the composition of the basal lamina between the astrocytic end-feet and the endothelial cells is only partly known. Here, we present a proteomic analysis of the ba ...[more]

PMID: 33226812

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