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Drosophila nephrocytes Par3A and B rescue

ABSTRACT: We assessed the functional role of the cell polarity proteins Par3A and B in Bazooka Knockdown nephrocytes. Here, we did a proteomics anaylsis of isolated garland nephrocytes from 3rd instar larvae. Analysis of the data identified differentially regulated proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Nephrocyte

SUBMITTER: Martin Hoehne

LAB HEAD: Paul Thomas Brinkkoetter

PROVIDER: PXD018636 | Pride | 2022-07-07

REPOSITORIES: Pride

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Scaffold polarity proteins Par3A and Par3B share redundant functions while Par3B acts independent of atypical protein kinase C/Par6 in podocytes to maintain the kidney filtration barrier.

Koehler Sybille S Odenthal Johanna J Ludwig Vivian V Unnersjö Jess David D Höhne Martin M Jüngst Christian C Grawe Ferdi F Helmstädter Martin M Janku Johanna L JL Bergmann Carsten C Hoyer Peter F PF Hagmann H Henning HH Walz Gerd G Bloch Wilhelm W Niessen Carien C Schermer Bernhard B Wodarz Andreas A Denholm Barry B Benzing Thomas T Iden Sandra S Brinkkoetter Paul T PT

Kidney international 20211217 4

Glomerular diseases are a major cause for chronic kidney disorders. In most cases podocyte injury is causative for disease development. Cytoskeletal rearrangements and morphological changes are hallmark features of podocyte injury and result in dedifferentiation and loss of podocytes. Here, we establish a link between the Par3 polarity complex and actin regulators necessary to establish and maintain podocyte architecture by utilizing mouse and Drosophila models to characterize the functional rol ...[more]

PMID: 34929254

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