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Activity-based protein profiling of the mouse gut microbiome in response to high or low fiber diets

ABSTRACT: Gastrointestinal content of mice fed high or low fiber diets was labeled with the GH2c-ABP. 3x replicates of each diet, 2x littermates per diet. 12 total mice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Environmental Samples

TISSUE(S): Gut

SUBMITTER: Matthew Monroe

LAB HEAD: Aaron Wright

PROVIDER: PXD018644 | Pride | 2022-08-11

REPOSITORIES: Pride

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Activity-based protein profiling identifies alternating activation of enzymes involved in the bifidobacterium shunt pathway or mucin degradation in the gut microbiome response to soluble dietary fiber.

Killinger Bryan J BJ Whidbey Christopher C Sadler Natalie C NC DeLeon Adrian J AJ Munoz Nathalie N Kim Young-Mo YM Wright Aaron T AT

NPJ biofilms and microbiomes 20220720 1

While deprivation of dietary fiber has been associated with adverse health outcomes, investigations concerning the effect of dietary fiber on the gut microbiome have been largely limited to compositional sequence-based analyses or utilize a defined microbiota not native to the host. To extend understanding of the microbiome's functional response to dietary fiber deprivation beyond correlative evidence from sequence-based analyses, approaches capable of measuring functional enzymatic activity are ...[more]

PMID: 35858888

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