Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration

ABSTRACT: This study sought to explore phosphoproteome signaling dysregulation in different models of neurodegeneration. We collected phosphotyrosine, MAPK/CDK substrates, global phosphoproteome, and protein expression data from hippocampus and/or cortex tissue from each model. Tryptic peptides were multiplexd with TMT, enriched for phosphopeptides, and then identified and quantified by LC-MS/MS. We additionally quantified phosphotyrosine peptides from BV-2 cells expressing various Siglec receptor constructs.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Nader Morshed  

LAB HEAD: Forest Michael White

PROVIDER: PXD018757 | Pride | 2020-11-09

REPOSITORIES: Pride