Targeted proteomics analysis of cortical collecting ducts with Pendrin knockout
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Kidney
SUBMITTER: 
Markus Rinschen
LAB HEAD: Markus Rinschen
PROVIDER: PXD018791 | Pride | 2022-04-04
REPOSITORIES: Pride
Publications
Viewing Cortical Collecting Duct Function Through Phenotype-guided Single-Tubule Proteomics.
Himmerkus Nina N Svendsen Samuel L SL Quintanova Catarina C Bleich Markus M Von Schwerdtner Otto O Benzing Thomas T Welling Paul A PA Leipziger Jens J Rinschen Markus M MM
Function (Oxford, England) 20200702 1
The revolution of the omics technologies has enabled profiling of the molecules of any sample. However, the heterogeneity of the kidney with highly specialized nephron segments like the cortical collecting duct (CCD) poses a challenge regarding integration of omics data and functional analysis. We examined function and proteome from the same single CCDs of C57Bl6 mice by investigating them in a double-barreled perfusion system before targeted mass spectrometry. Transepithelial voltage (V<sub>te< ...[more]
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