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Phospho-proteomic data on the LATS1 interactome from cells treated with two proapoptotic signals: FAS and etoposide

ABSTRACT: Using this approach, we obtained phospho-proteomic data on the LATS1 interactome from cells treated with two proapoptotic signals: FAS and etoposide, which both activate LATS kinase activity [28].To identify the LATS1 interactome we transiently expressed GFP-LATS1 in HeLa cells, immunoprecipitated GFP-LATS1 with anti-GFP antibodies and identified the associated proteins using mass-spectrometry. Unspecific binding proteins were discarded by comparing with the control GFP IP.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cervical Cell Line

DISEASE(S): Cervical Cancer

SUBMITTER: David Matallanas

LAB HEAD: David Gomez

PROVIDER: PXD018903 | Pride | 2021-09-09

REPOSITORIES: Pride

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IQGAP1 Is a Scaffold of the Core Proteins of the Hippo Pathway and Negatively Regulates the Pro-Apoptotic Signal Mediated by This Pathway.

Quinn Niall P NP García-Gutiérrez Lucía L Doherty Carolanne C von Kriegsheim Alexander A Fallahi Emma E Sacks David B DB Matallanas David D

Cells 20210223 2

The Hippo pathway regulates a complex signalling network which mediates several biological functions including cell proliferation, organ size and apoptosis. Several scaffold proteins regulate the crosstalk of the members of the pathway with other signalling pathways and play an important role in the diverse output controlled by this pathway. In this study we have identified the scaffold protein IQGAP1 as a novel interactor of the core kinases of the Hippo pathway, MST2 and LATS1. Our results ind ...[more]

PMID: 33672268

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