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RNF41 regulation of the dendritic cell receptor Clec9A

ABSTRACT: The dendritic cell receptor Clec9A facilitates processing of dead cell-derived antigens for cross-presentation and the induction of effective CD8+ T cell immune responses. This study investigates the role of E3 ubiquitin ligase RNF41 in Clec9A-mediated ubiquitination, and Clec9A-interactions.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ralf Schittenhelm

LAB HEAD: Mireille Lahoud

PROVIDER: PXD018926 | Pride | 2020-11-27

REPOSITORIES: Pride

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RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.

Tullett Kirsteen M KM Tan Peck Szee PS Park Hae-Young HY Schittenhelm Ralf B RB Michael Nicole N Li Rong R Policheni Antonia N AN Gruber Emily E Huang Cheng C Fulcher Alex J AJ Danne Jillian C JC Czabotar Peter E PE Wakim Linda M LM Mintern Justine D JD Ramm Georg G Radford Kristen J KJ Caminschi Irina I O'Keeffe Meredith M Villadangos Jose A JA Wright Mark D MD Blewitt Marnie E ME Heath William R WR Shortman Ken K Purcell Anthony W AW Nicola Nicos A NA Zhang Jian-Guo JG Lahoud Mireille H MH

eLife 20201202

The dendritic cell receptor Clec9A facilitates processing of dead cell-derived antigens for cross-presentation and the induction of effective CD8<sup>+</sup> T cell immune responses. Here, we show that this process is regulated by E3 ubiquitin ligase RNF41 and define a new ubiquitin-mediated mechanism for regulation of Clec9A, reflecting the unique properties of Clec9A as a receptor specialized for delivery of antigens for cross-presentation. We reveal RNF41 is a negative regulator of Clec9A and ...[more]

PMID: 33264090

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