Proteomics

Dataset Information

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Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules


ABSTRACT: Chemical biology strategies for directly perturbing protein homeostasis including the degradation tag (dTAG) system provide temporal advantages over genetic approaches and improved selectivity over small molecule inhibitors. We describe dTAGV-1, an exclusively selective VHL-recruiting dTAG molecule, to rapidly degrade FKBP12F36V-tagged proteins. dTAGV-1 overcomes a limitation of previously reported CRBN-recruiting dTAG molecules to degrade recalcitrant oncogenes, supports combination degrader studies and facilitates investigations of protein function in cells and mice

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo sapiens  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Disease Free

SUBMITTER: Behnam Nabet  

LAB HEAD: Nathanael Gray

PROVIDER: PXD018937 | Pride | 2020-09-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EWS-FLI-FKBP12_dTAG_f1.mzIdentML Mzid
EWS-FLI-FKBP12_dTAG_f1.raw Raw
EWS-FLI-FKBP12_dTAG_f10.mzIdentML Mzid
EWS-FLI-FKBP12_dTAG_f10.raw Raw
EWS-FLI-FKBP12_dTAG_f11.mzIdentML Mzid
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Publications


Chemical biology strategies for directly perturbing protein homeostasis including the degradation tag (dTAG) system provide temporal advantages over genetic approaches and improved selectivity over small molecule inhibitors. We describe dTAG<sup>V</sup>-1, an exclusively selective VHL-recruiting dTAG molecule, to rapidly degrade FKBP12<sup>F36V</sup>-tagged proteins. dTAG<sup>V</sup>-1 overcomes a limitation of previously reported CRBN-recruiting dTAG molecules to degrade recalcitrant oncogenes,  ...[more]

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