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Thermococcus eurythermalis A501, high hydrostatic pressure

ABSTRACT: In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of pressure adaptation: HP (40 MPa) tested under 85°C and 95°C, and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 10 MPa) was used as the control.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Thermococcus Eurythermalis

SUBMITTER: weishu zhao

LAB HEAD: Xiao Xiang

PROVIDER: PXD019007 | Pride | 2020-09-09

REPOSITORIES: Pride

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Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.

Zhao Weishu W Ma Xiaopan X Liu Xiaoxia X Jian Huahua H Zhang Yu Y Xiao Xiang X

Frontiers in microbiology 20200910

Hyperthermophiles, living in environments above 80°C and usually coupling with multi-extreme environmental stresses, have drawn great attention due to their application potential in biotechnology and being the primitive extant forms of life. Studies on their survival and adaptation mechanisms have extended our understanding on how lives thrive under extreme conditions. During these studies, the "cross-stress" behavior in various organisms has been observed between the extreme high temperature an ...[more]

PMID: 33013758

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Thermococcus eurythermalis A501, Temperature adaptation

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of temperature adaptation: low temperature (65°C) and high temperature (95°C), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

2020-09-09 | PXD018991 | Pride

Thermococcus eurythermalis A501 pH adaptation

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of pH adaptation: low pH (pH 4) and high pH (pH 9), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

2020-09-09 | PXD018974 | Pride

Thermococcus eurythermalis A501, NaCl adaptation

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of NaCl adaptation: low salinity (1.5% NaCl, w/v) and high salinity (4.5% NaCl, w/v), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

2020-09-09 | PXD018994 | Pride

Mouse oocytes: High hydrostatic pressure (HP) treated vs. Control

Project description:Transcription profiling of mouse oocytes treated with 20 MPa hydrostatic pressure for 60 minutes at 37 °C comparing control oocytes kept under identical conditions as pressure treated ones, except HHP treatment. One-condition experiment, HP treated oocytes vs. Control oocytes. Biological replicates: 4 HP treated replicates, 4 control replicates.

2012-01-06 | E-GEOD-28410 | biostudies-arrayexpress

Proteomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in Synechocystis sp. PCC 6803

Project description:Using an engineered 3-HP producing SM strain, in this project, the cellular responses to 3-HP internally produced were first determined using a quantitative iTRAQ-LC-MS/MS proteomics approach. A total of 2,264 unique proteins were identified, which represented about 63% of all predicted protein in Synechocystis in the proteomic analysis of the 3-HP producing strain SM and wild type. Among all identified proteins, 204 proteins were found up- regulated and 123 proteins were found down-regulated, respectively. The proteins related to oxidative phosphorylation, photosynthesis, ribosome, central carbon metabolism, two-component systems and ABC-type transporters were up-regulated. The results suggested that the supply of ATP and NADPH was increased significantly, and the precursor malonyl-CoA and acetyl-CoA may also be supplemented when 3-HP was produced at a high level in Synechocystis.

2016-09-26 | PXD004974 | Pride

Proteomic analysis of the immune response to dsRNA in B.dorsalis

Project description:Proteomic analysis of a mixed sample from 8, 12 and 24 h (early set) post first exposure to dsRNA and a sample from 5 days (late set) post first exposure to dsRNA to identify differentially expressed proteins which were responsible to the immune response to dsRNA in B.dorsalis.

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Ctenopharyngodon idella iTRAQ - Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

Project description:Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

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Mouse in vitro fertilized four-cell stage embryos: High hydrostatic pressure (HP) treated vs. Control

Project description:Mouse oocytes were treated with 20 MPa hydrostatic pressure for 60 minutes at 37 °C. The HHP treated oocytes and their untreated controls were fertilized by ICSI and cultured till four-cell stage, when the transcription profiling experiment was performed. One-condition experiment, Four-cell stage embryos from HP treated oocytes vs. four-cell stage embryos from control oocytes. Biological replicates: 3 HP treated replicates, 3 control replicates.

2012-01-06 | E-GEOD-28411 | biostudies-arrayexpress

myocardial tissue from atrial fibrillation patients

Project description:Electrical and structural remodeling processes are contributors to the self-perpetuating nature of atrial fibrillation (AF). However, their correlation has not been clarified. In this study, human atrial tissues from the patients with rheumatic mitral valve disease in either sinus rhythm or persistent AF were analyzed using a combined transcriptomic and proteomic approach. An up-regulation in chloride intracellular channel (CLIC) 1, 4, 5 and a rise in type IV collagen were revealed. Combined with the results from immunohistochemistry and electron microscope analysis, the distribution of type IV collagen and effects of fibrosis on myocyte membrane indicated the possible interaction between CLIC and type IV collagen, confirmed by protein structure prediction and co-immunoprecipitation. These results indicate that CLICs play an important role in the development of atrial fibrillation and that CLICs and structural type IV collagen may interact on each other to promote the development of AF in rheumatic mitral valve disease.

2018-11-22 | PXD006911 | Pride

Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology

Project description:Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve the allograft function. Here, we applied isobaric tags for relative and absolute quantisation (iTRAQ) mass spectrometry to analyse serum protein expression in AR patients and healthy controls. Overall, 1399 proteins were identified. Using a cut-off of Q<0.05 and a fold change > 1.2 for the expressed variation, 109 proteins showed distinct differential expression between the AR and control groups, of whom 72 were upregulated and 37 were downregulated. Several proteins, such as properdin, keratin 1, lipoprotein (a) and vitamin D-binding protein, may play roles in the pathogenesis of AR. This study focused on iTRAQ-based proteomic profiling of serum samples in AR. Insights from our work might help advance our understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.

2021-09-08 | PXD015336 | Pride

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