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Identification of RNG140 (caprin2)-associated proteins in CHO cells

ABSTRACT: Regulation of gene expression at the translational level is key to determining cell fate and function. An RNA-binding protein RNG140 (caprin2) plays a role in cell differentiation such as eye lens development and has been reported to function in translational regulation. To identify RNG140-associated proteins, we generated CHO cells stably expressing GFP and RNG140-GFP, and performed immunoprecipitation (IP) with GFP antibody from those cells followed by mass spectrometry. We identified about 400 proteins specifically co-immunoprecipitated with RNG140-GFP but not with GFP. Gene ontology (GO) enrichment analysis revealed that the eIF3 subunit proteins and small ribosomal subunit proteins were significantly enriched in the RNG140-associated complex.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Epithelial Cell

SUBMITTER: Kaori Nakazawa

LAB HEAD: Nobuyuki Shiina

PROVIDER: PXD019040 | Pride | 2020-08-17

REPOSITORIES: Pride

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