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A2M Q975C and C3 Q1013C disulfide mutants

ABSTRACT: Mutant A2M and C3 with disulfides replacing their thiol esters were disulfide mapped via pepsin digests.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Seandean Harwood

LAB HEAD: Jan J. Enghild

PROVIDER: PXD019082 | Pride | 2021-02-18

REPOSITORIES: Pride

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Substituting the Thiol Ester of Human A2M or C3 with a Disulfide Produces Native Proteins with Altered Proteolysis-Induced Conformational Changes.

Harwood Seandean Lykke SL Nielsen Nadia Sukusu NS Pedersen Henrik H Kjøge Katarzyna K Nielsen Peter Kresten PK Andersen Gregers Rom GR Enghild Jan J JJ

Biochemistry 20201210 51

Most proteins in the α-macroglobulin (αM) superfamily contain reactive thiol esters that are required for their biological function. Here, we have characterized the human α2-macroglobulin (A2M) and complement component C3 mutants A2M Q975C and C3 Q1013C, which replace the CGEQ thiol ester motifs of the original proteins with the disulfide-forming sequence CGEC. Mass spectrometry showed that the intended disulfide was formed in both proteins. The correct folding and native conformation of A2M Q97 ...[more]

PMID: 33301305

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