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Proline hydroxylation of ChREBP

ABSTRACT: This project investigated proline hydroxylation of ChREBP. Proline hydroxylation was investigated in flag-IP enriched protein extracts from ChREBP-flag overexpressing HEK293 cells (A) and in ChREBP-IP enriched mouse liver protein (male, C57BL6/J) (B).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Michael Schupp

LAB HEAD: Michael Schupp

PROVIDER: PXD019137 | Pride | 2020-10-19

REPOSITORIES: Pride

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The glucose-sensing transcription factor ChREBP is targeted by proline hydroxylation.

Heidenreich Steffi S Weber Pamela P Stephanowitz Heike H Petricek Konstantin M KM Schütte Till T Oster Moritz M Salo Antti M AM Knauer Miriam M Goehring Isabel I Yang Na N Witte Nicole N Schumann Anne A Sommerfeld Manuela M Muenzner Matthias M Myllyharju Johanna J Krause Eberhard E Schupp Michael M

The Journal of biological chemistry 20201006 50

Cellular energy demands are met by uptake and metabolism of nutrients like glucose. The principal transcriptional regulator for adapting glycolytic flux and downstream pathways like <i>de novo</i> lipogenesis to glucose availability in many cell types is carbohydrate response element-binding protein (ChREBP). ChREBP is activated by glucose metabolites and post-translational modifications, inducing nuclear accumulation and regulation of target genes. Here we report that ChREBP is modified by prol ...[more]

PMID: 33023907

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