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Quantitative proteomic analysis of proteasome in response to MG132

ABSTRACT: The ubiquitin-proteasome system and autophagy are two major intracellular proteolytic pathways and both remove misfolded and proteotoxic proteins from eukaryotic cells. This study describes the detailed informations about the change in composition and interactome of proteasomes after prolonged MG132 treatment.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Cell, Kidney

SUBMITTER: Dohyun Han

LAB HEAD: Dohyun Han

PROVIDER: PXD019193 | Pride | 2021-09-09

REPOSITORIES: Pride

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Aggresomal sequestration and STUB1-mediated ubiquitylation during mammalian proteaphagy of inhibited proteasomes.

Choi Won Hoon WH Yun Yejin Y Park Seoyoung S Jeon Jun Hyoung JH Lee Jeeyoung J Lee Jung Hoon JH Yang Su-A SA Kim Nak-Kyoon NK Jung Chan Hoon CH Kwon Yong Tae YT Han Dohyun D Lim Sang Min SM Lee Min Jae MJ

Proceedings of the National Academy of Sciences of the United States of America 20200728 32

The 26S proteasome, a self-compartmentalized protease complex, plays a crucial role in protein quality control. Multiple levels of regulatory systems modulate proteasomal activity for substrate hydrolysis. However, the destruction mechanism of mammalian proteasomes is poorly understood. We found that inhibited proteasomes are sequestered into the insoluble aggresome via HDAC6- and dynein-mediated transport. These proteasomes colocalized with the autophagic receptor SQSTM1 and cleared through sel ...[more]

PMID: 32723828

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