Dataset Information

TGF-Beta-induced Dact1 Biomolecular Condensates Repress Wnt Signaling via Multivalent Protein-Protein Interactions

ABSTRACT: Isolation and characterization of Dact1 biomolecular condensates with and without Wnt stimulation in breast cancer bone metastatic cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Bone Marrow

DISEASE(S): Breast Cancer

SUBMITTER: Katelyn Cook

LAB HEAD: Cristea, Ileana

PROVIDER: PXD019271 | Pride | 2021-01-18

REPOSITORIES: Pride

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			Action	DRS
	20190906_Mark_Dact1_DOC_84C.raw	Raw
	20190906_Mark_Dact1_DOC_84C_1.raw	Raw
	20190906_Mark_Dact1_DOC_84C_2.raw	Raw
	20190906_Mark_Dact1_DOC_84W.raw	Raw
	20190906_Mark_Dact1_DOC_84W_1.raw	Raw

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Project description:Tumor: tumor microenvironment (TME) interactions are critical for tumor progression and the composition and structure of the local extracellular matrix (ECM) are key determinants of tumor metastasis. We recently reported that activation of Wnt/beta- catenin signaling in Ewing sarcoma cells induces widespread transcriptional changes that are associated with acquisition of a metastatic tumor phenotype. Significantly, ECM protein-encoding genes were found to be enriched among Wnt/beta-catenin induced transcripts, leading us to hypothesize that activation of canonical Wnt signaling might induce changes in the Ewing sarcoma secretome. To address this hypothesis, conditioned media from Ewing sarcoma cell lines cultured in the presence or absence of Wnt3a was collected for proteomic analysis. Label-free mass spectrometry was used to identify and quantify differentially secreted proteins. We then used in silico databases to identify only proteins annotated as secreted. Comparison of the secretomes of two Ewing sarcoma cell lines revealed numerous shared proteins, as well as a degree of heterogeneity, in both basal and Wnt-stimulated conditions. Gene set enrichment analysis of secreted proteins revealed that Wnt stimulation reproducibly resulted in increased secretion of proteins involved in ECM organization, ECM receptor interactions, and collagen formation. In particular, Wnt-stimulated Ewing sarcoma cells upregulated secretion of structural collagens, as well as matricellular proteins, such as the metastasis-associated protein, tenascin C (TNC). Interrogation of published databases confirmed reproducible correlations between Wnt/beta-catenin activation and TNC and COL1A1 expression in patient tumors. In summary, this first study of the Ewing sarcoma secretome reveals that Wnt/beta-catenin activated tumor cells upregulate secretion of ECM proteins. Such Wnt/beta-catenin mediated changes are likely to impact on tumor: TME interactions that contribute to metastatic progression.

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TGF-Beta-induced Dact1 Biomolecular Condensates Repress Wnt Signaling via Multivalent Protein-Protein Interactions

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