Proteomics

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Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype


ABSTRACT: The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. A total of 1,976 proteins have been identified using public databases. One hundred ninety six proteins were found significantly differentially expressed; results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. This comparison selected thirty six proteins as discriminant biomarkers among A. simplex, A. pegreffii and their hybrid genotype; eighteen of these biomarkers, encoded by nine loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12 and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Anisakis Simplex Complex

SUBMITTER: Sergio Ciordia  

LAB HEAD: Alfonso Navas

PROVIDER: PXD019289 | Pride | 2020-08-17

REPOSITORIES: Pride

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Publications

Quantitative Proteomics Comparison of Total Expressed Proteomes of <i>Anisakis simplex</i> Sensu Stricto, <i>A. pegreffii</i>, and Their Hybrid Genotype.

C Arcos Susana S   Robertson Lee L   Ciordia Sergio S   Sánchez-Alonso Isabel I   Careche Mercedes M   Carballeda-Sanguiao Noelia N   Gonzalez-Muñoz Miguel M   Navas Alfonso A  

Genes 20200810 8


The total proteomes of <i>Anisakis simplex</i> s.s., <i>A. pegreffii</i> and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. Comparison was made by means of two independent experiments considering four biological replicates of <i>A. simplex</i> and two each for <i>A. pegreffii</i> and hybrid between both species. A total of 1811 and 1976 proteins have been respectively identified in the experiments using publi  ...[more]

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