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CTCF loss has limited effects on genome architecture in Drosophila despite critical regulatory functions.

ABSTRACT: Proteins co-purifying with recombinantly purified and N-terminally GFP-3C-tagged CTCF N-terminus (amino acids 1-293) produced in bacteria were identified by incubating the tagged bait with soluble nuclear protein extracts from 0-12h hour-old wildtype (OregonR strain) Drosophila melanogaster embryos.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Manfredo Quadroni

LAB HEAD: Maria Cristina Gambetta

PROVIDER: PXD019487 | Pride | 2021-01-18

REPOSITORIES: Pride

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CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions.

Kaushal Anjali A Mohana Giriram G Dorier Julien J Özdemir Isa I Omer Arina A Cousin Pascal P Semenova Anastasiia A Taschner Michael M Dergai Oleksandr O Marzetta Flavia F Iseli Christian C Eliaz Yossi Y Weisz David D Shamim Muhammad Saad MS Guex Nicolas N Lieberman Aiden Erez E Gambetta Maria Cristina MC

Nature communications 20210212 1

Vertebrate genomes are partitioned into contact domains defined by enhanced internal contact frequency and formed by two principal mechanisms: compartmentalization of transcriptionally active and inactive domains, and stalling of chromosomal loop-extruding cohesin by CTCF bound at domain boundaries. While Drosophila has widespread contact domains and CTCF, it is currently unclear whether CTCF-dependent domains exist in flies. We genetically ablate CTCF in Drosophila and examine impacts on genome ...[more]

PMID: 33579945

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