Microwave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride

ABSTRACT: We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis using bottom-up approach. Our methodology shows high labelling efficiency and demonstrates its utilization for histone posttranslational modification abundance monitoring. Therefore, to be an alternative to conventionally used methods.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Culture

SUBMITTER: Hana Kucharikova  

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD019502 | Pride | 2021-06-14

REPOSITORIES: Pride