A primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): T Cell, Blood
SUBMITTER: 
Harshi Weerakoon
LAB HEAD: Michelle Hill
PROVIDER: PXD019542 | Pride | 2020-10-19
REPOSITORIES: Pride
Publications
A primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics.
Weerakoon Harshi H Potriquet Jeremy J Shah Alok K AK Reed Sarah S Jayakody Buddhika B Kapil Charu C Midha Mukul K MK Moritz Robert L RL Lepletier Ailin A Mulvenna Jason J Miles John J JJ Hill Michelle M MM
Scientific data 20201123 1
Data independent analysis (DIA) exemplified by sequential window acquisition of all theoretical mass spectra (SWATH-MS) provides robust quantitative proteomics data, but the lack of a public primary human T-cell spectral library is a current resource gap. Here, we report the generation of a high-quality spectral library containing data for 4,833 distinct proteins from human T-cells across genetically unrelated donors, covering ~24% proteins of the UniProt/SwissProt reviewed human proteome. SWATH ...[more]
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