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Confirmation of recombinant Calcium-activated potassium channel from Leishmania donovani

ABSTRACT: Calcium-activated potassium channels are important membrane proteins that help in maintaining the cellular physiology and cell signalling. Till date no Calcium-activated potassium channels have been identified in Leishmania donovani. It’s gene was cloned into pET vector and expressed in E. coli BL21 (DE3). The recombinant protein was purified using nickel affinity chromatography.

INSTRUMENT(S):

ORGANISM(S): Leishmania Donovani Leishmania Sp. Mhom/in/80/dd8

DISEASE(S): Visceral Leishmaniasis

SUBMITTER: ANINDITA PAUL

LAB HEAD: Dr. Sushma Singh

PROVIDER: PXD019577 | Pride | 2021-05-11

REPOSITORIES: Pride

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			Action	DRS
	B8_MS_1.pdf	Pdf
	FASTA.fasta	Fasta
	FTeeiaemE.csv	Csv
	FTeeiaemE.fasta	Fasta
	FTeeiaemE.mgf	Mgf

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Publications

Identification of a novel calcium activated potassium channel from Leishmania donovani and in silico predictions of its antigenic features.

Paul Anindita A Mubashra Singh Sushma S

Acta tropica 20210418

Visceral Leishmaniasis is a major neglected tropical disease with increasing incidences of drug resistance. This has led to the search for a suitable drug target for chemotherapeutic intervention. Potassium channels are a family of membrane proteins which play a vital role in homeostasis and any perturbation in them alters cell survival which makes them an attractive target. To characterize a calcium-activated potassium channel from Leishmania donovani (LdK<sub>Ca</sub>), a putative ion-channel ...[more]

PMID: 33878308

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