Proteomics

Dataset Information

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Confirmation of recombinant Calcium-activated potassium channel from Leishmania donovani


ABSTRACT: Calcium-activated potassium channels are important membrane proteins that help in maintaining the cellular physiology and cell signalling. Till date no Calcium-activated potassium channels have been identified in Leishmania donovani. It’s gene was cloned into pET vector and expressed in E. coli BL21 (DE3). The recombinant protein was purified using nickel affinity chromatography.

INSTRUMENT(S): 4700 Proteomics Analyzer

ORGANISM(S): Leishmania Donovani Leishmania Sp. Mhom/in/80/dd8

DISEASE(S): Visceral Leishmaniasis

SUBMITTER: ANINDITA PAUL  

LAB HEAD: Dr. Sushma Singh

PROVIDER: PXD019577 | Pride | 2021-05-11

REPOSITORIES: Pride

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Publications

Identification of a novel calcium activated potassium channel from Leishmania donovani and in silico predictions of its antigenic features.

Paul Anindita A   Mubashra   Singh Sushma S  

Acta tropica 20210418


Visceral Leishmaniasis is a major neglected tropical disease with increasing incidences of drug resistance. This has led to the search for a suitable drug target for chemotherapeutic intervention. Potassium channels are a family of membrane proteins which play a vital role in homeostasis and any perturbation in them alters cell survival which makes them an attractive target. To characterize a calcium-activated potassium channel from Leishmania donovani (LdK<sub>Ca</sub>), a putative ion-channel  ...[more]

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