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Wobble tRNA modification dependent proteome

ABSTRACT: We want to assess the features of proteins whose translation depends on the mcm5s2 modification of urifdine in position 34 of some tRNAs.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Francesca Rapino

LAB HEAD: Pierre CLOSE

PROVIDER: PXD019620 | Pride | 2021-02-24

REPOSITORIES: Pride

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Wobble tRNA modification and hydrophilic amino acid patterns dictate protein fate.

Rapino Francesca F Zhou Zhaoli Z Roncero Sanchez Ana Maria AM Joiret Marc M Seca Christian C El Hachem Najla N Valenti Gianluca G Latini Sara S Shostak Kateryna K Geris Liesbet L Li Ping P Huang Gang G Mazzucchelli Gabriel G Baiwir Dominique D Desmet Christophe J CJ Chariot Alain A Georges Michel M Close Pierre P

Nature communications 20210415 1

Regulation of mRNA translation elongation impacts nascent protein synthesis and integrity and plays a critical role in disease establishment. Here, we investigate features linking regulation of codon-dependent translation elongation to protein expression and homeostasis. Using knockdown models of enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), we show that gene codon content is necessary but not sufficient to predict protein fate. ...[more]

PMID: 33859181

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