Proteomics

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Regulation of phospholipid synthesis and vesicular trafficking in Toxoplasma gondii by protein kinase TgCDPK7

ABSTRACT: Calcium Dependent Protein Kinases (CDPKs) are key effector in calcium signaling in Apicomplexan parasites. CDPK7 is one such kinase, which plays a crucial role in intracellular infection of Toxoplasma gondii parasite. To gain insights into how CDPK7 play a role in parasites survival, we have performed comparative phosphoproteomic to identify putative targets of TgCDPK7 in the parasites. Proteins from TgCDPK7 knockdown and wild were isolated, digested with trypsin and labelled with TMT. From the quantitative proteomic analysis by high- resolution mass spectrometry, differentially phosphorylated proteins were identified, which can be direct or indirect substrates of TgCDPK7. Many proteins which are involved in lipid metabolism are also found significantly altered.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Keshava Prasad T. S.  

LAB HEAD: Dr. T. S. Keshava Prasad

PROVIDER: PXD019655 | Pride | 2021-04-27

REPOSITORIES: Pride

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Protein kinase TgCDPK7 regulates vesicular trafficking and phospholipid synthesis in Toxoplasma gondii.

Bansal Priyanka P   Antil Neelam N   Kumar Manish M   Yamaryo-Botté Yoshiki Y   Rawat Rahul Singh RS   Pinto Sneha S   Datta Keshava K KK   Katris Nicholas J NJ   Botté Cyrille Y CY   Prasad T S Keshava TSK   Sharma Pushkar P  

PLoS pathogens 20210226 2

Apicomplexan parasites are causative agents of major human diseases. Calcium Dependent Protein Kinases (CDPKs) are crucial components for the intracellular development of apicomplexan parasites and are thus considered attractive drug targets. CDPK7 is an atypical member of this family, which initial characterization suggested to be critical for intracellular development of both Apicomplexa Plasmodium falciparum and Toxoplasma gondii. However, the mechanisms via which it regulates parasite replic  ...[more]

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