Dataset Information

Quantitative proteomics identifies Yashtimadhu as a neuroprotectant against rotenone induced cellular model of Parkinson’s disease

ABSTRACT: We aimed to explore the neuroprotective efficacy of Yashtimadhu powder, prepared from the roots of Glycyrrhiza glabra L. (https://mpns.science.kew.org; http://www.ayurveda.hu/api/API-Vol-1.pdf) commonly known as licorice, a known Medhya rasayana in the Indian Ayurvedic texts against Parkinson’s disease. To test the efficacy of Yashtimadhu powder, we used rotenone to generate an in vitro PD model using retinoic acid-differentiated IMR32 cells. Yashtimadhu powder (Lot No.64) was procured from SDP Remedies and Research Centre, Mangalore, India, a GMP-certified Ayurvedic product manufacturer ((http://sdpayurveda.com/products/choorna/yastimadhu-choorna/)The Yashtimadhu neuroprotection was ascertained using cell cytotoxicity, activation of apoptotic proteins and kinases. We used Tandem mass tag (TMT)-lables to understand the differentially regulated proteins across various treatment groups, using Orbitrap Fusion Tribrid mass spectrometer. The mass spectrometry data is acquired in biological duplicates and technical triplicates. We report 4,864 proteins corresponding to 4,783 gene symbols. The proteins were filtered out based technical and biological replicate concordance, thereby reporting 3,720 proteins common among the biological replicates.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Imr-32 Cell

DISEASE(S): Parkinson's Disease

SUBMITTER: Keshava Prasad T. S.

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD019672 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Identification of Molecular Network Associated with Neuroprotective Effects of Yashtimadhu (Glycyrrhiza glabra L.) by Quantitative Proteomics of Rotenone-Induced Parkinson's Disease Model.

Karthikkeyan Gayathree G Najar Mohd Altaf MA Pervaje Ravishankar R Pervaje Sameera Krishna SK Modi Prashant Kumar PK Prasad Thottethodi Subrahmanya Keshava TSK

ACS omega 20201006 41

Parkinson's disease (PD) is a progressive neurodegenerative disorder, whose treatment with modern therapeutics leads to a plethora of side effects with prolonged usage. Therefore, the management of PD with complementary and alternative medicine is often pursued. In the Ayurveda system of alternative medicine, Yashtimadhu choorna, a Medhya Rasayana (nootropic), prepared from the dried roots of Glycyrrhiza glabra L. (licorice), is prescribed for the management of PD with a favorable ...[more]

PMID: 33110989

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Dataset Information

Quantitative proteomics identifies Yashtimadhu as a neuroprotectant against rotenone induced cellular model of Parkinson’s disease

Publications

Identification of Molecular Network Associated with Neuroprotective Effects of Yashtimadhu (<i>Glycyrrhiza glabra</i> L.) by Quantitative Proteomics of Rotenone-Induced Parkinson's Disease Model.

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