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Aurora-A interactome in MV4-11 cells

ABSTRACT: To determine interactors of Aurora-A, HA tagged Aurora-A was immunoprecipitated from MV4-11 cells stably expressing HA tagged Aurora-A wild type and compared to MV4-11 cells expressing empty vector.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Bikash Adhikari

LAB HEAD: Elmar Wolf

PROVIDER: PXD019684 | Pride | 2020-09-28

REPOSITORIES: Pride

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			Action	DRS
	2019-12-10_Fusion_20269.raw	Raw
	2019-12-10_Fusion_20270.raw	Raw
	2019-12-10_Fusion_20271.raw	Raw
	2019-12-10_Fusion_20272.raw	Raw
	2019-12-10_Fusion_20273.raw	Raw

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Proteomics analysis with the Aurora-A PROTAC, JB170

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Comparation the mRNA Expression Profile of the MV4-11 Sorafenib Resistant Cells and the Parental Cells

Project description:Our gene set analysis of MV4-11-R versus MV4-11 indicated decreased depolarization of mitochondria and mitochondrial membrane, mitochondrial dysfunction and anti-apoptosis as other top ranked molecular or cellular functions of differential gene sets. expression of most genes encoding glycolytic enzymes was up-regulated in MV4-11-R cells we revealed a metabolic alteration in sorafenib-resistant cell lines with mitochondrial respiration deficiency, leading to substantial decrease of mitochondria-derived ATP generation and a significant increase in glycolytic activity to maintain sufficient ATP production. Our study revealed a metabolic signature of sorafenib resistance and indicated that increase of glycolytic activity including upregulation of major glycolytic enzymes may be viewed as a marker for early detection of sorafenib resistance in AML patients with FLT3/ITD mutation and glycolytic inhibitors warrant further investigation as alternative therapeutic agents for sorafenib-resistant cells Sorafenib resistant cells MV411-R VS. parental MV4-11 cells. Biological replicates: 3 control replicates, 3 treated replicates.

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