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SmD3 protein interactome in Drosophila melanogaster wing imaginal discs

ABSTRACT: Screening for binding partners of the splicing factor SmD3 and changes in interaction upon depletion of the protein Ecdysoneless (Ecd) in the nubbin domain of third-instar larval wing imaginal discs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster Drosophila Melanogaster (fruit Fly)

TISSUE(S): Wing Disc

SUBMITTER: Steffen Erkelenz

LAB HEAD: Mirka Uhlirova

PROVIDER: PXD019761 | Pride | 2021-09-09

REPOSITORIES: Pride

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Ecd promotes U5 snRNP maturation and Prp8 stability.

Erkelenz Steffen S Stanković Dimitrije D Mundorf Juliane J Bresser Tina T Claudius Ann-Katrin AK Boehm Volker V Gehring Niels H NH Uhlirova Mirka M

Nucleic acids research 20210201 3

Pre-mRNA splicing catalyzed by the spliceosome represents a critical step in the regulation of gene expression contributing to transcriptome and proteome diversity. The spliceosome consists of five small nuclear ribonucleoprotein particles (snRNPs), the biogenesis of which remains only partially understood. Here we define the evolutionarily conserved protein Ecdysoneless (Ecd) as a critical regulator of U5 snRNP assembly and Prp8 stability. Combining Drosophila genetics with proteomic approaches ...[more]

PMID: 33444449

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