Proteomics

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Proteomic study of avocado seed tissues (testa, cotyledon, and embryo) during postharvest shelf life

ABSTRACT: In this study, we scrutinized by comparative proteomics using Tandem Mass Tags (TMT) couple to synchronous-precursor selection (SPS)-MS3 the dynamic of the proteome of specific tissues of avocado seeds (testa, cotyledon, and embryo) during postharvest shelf life. Our proteomics pipeline allows the identification of more proteins in testa compared to other tissues, and also, we could track the dynamic of more protein in this tissue during PSL. In general, proteins associated with stress, glycolic process, and starch metabolism were commonly identify in each tissue. In contracts, a drastic difference in protein translation, amino acid, and fatty acid metabolism was observed among tissues during PSL.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Persea Americana

TISSUE(S): Testis, Plant Cell, Embryo, Cotyledon

SUBMITTER: Eliel Ruiz-May  

LAB HEAD: Eliel Ruiz-May

PROVIDER: PXD019772 | Pride | 2021-03-18

REPOSITORIES: Pride

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Avocado is a nutritious and economically important fruit, generating significant income for exporter countries. Recently, by-products of this fruit such as seeds and peels, have raised interest in different industries. However, the biochemical features of the nutraceutical value of these tissues have not been analyzed using molecular approaches during the postharvest shelf life (PSL). We carried out comparative proteomics using tandem mass tagging (TMT) and synchronous-precursor selection (SPS)-  ...[more]

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