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Scorpion (Centruroides vittatus) venom gland

ABSTRACT: proteomic analysis of venom gland peptides from the scorpion Centruroides vittatus

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Centruroides Vittatus

TISSUE(S): Diploid Cell, Telson

DISEASE(S): Disease Free

SUBMITTER: tsunemi yamashita

LAB HEAD: Tsunemi Yamashita

PROVIDER: PXD019850 | Pride | 2022-02-15

REPOSITORIES: Pride

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Reduced Toxicity of <i>Centruroides vittatus</i> (Say, 1821) May Result from Lowered Sodium β Toxin Gene Expression and Toxin Protein Production.

Bowman Aimee A Fitzgerald Chloe C Pummill Jeff F JF Rhoads Douglas D DD Yamashita Tsunemi T

Toxins 20211122 11

Body tissue and venom glands from an eastern population of the scorpion <i>Centruroides vittatus</i> (Say, 1821) were homogenized and molecular constituents removed to characterize putative sodium β toxin gene diversity, RT-qPCR, transcriptomic, and proteomic variation. We cloned sodium β toxins from genomic DNA, conducted RT-qPCR experiments with seven sodium β toxin variants, performed venom gland tissue RNA-seq, and isolated venom proteins for mass spectrophotometry. We identified >70 putativ ...[more]

PMID: 34822614

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