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OTUD5 complex mass spectrometry

ABSTRACT: DUBs are involved in various pathways and signalling networks by pairing with E3 ubiquitin ligases in protein complexes. In order to test whether OTUD5 associates with an E3 ubiquitin ligase to regulate cell proliferation, the stable Flag-OTUD5/Hep3B cell line was established by transfecting Hep3B cells with plasmids expressing pCIN4-Flag-OTUD5. The cellular factors interacting with OTUD5 were purified by anti-Flag antibody-conjugated M2 beads.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Fangzhou Li

LAB HEAD: Fangzhou Li

PROVIDER: PXD019907 | Pride | 2020-09-11

REPOSITORIES: Pride

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OTUD5 cooperates with TRIM25 in transcriptional regulation and tumor progression via deubiquitination activity.

Li Fangzhou F Sun Qianqian Q Liu Kun K Zhang Ling L Lin Ning N You Kaiqiang K Liu Mingwei M Kon Ning N Tian Feng F Mao Zebin Z Li Tingting T Tong Tanjun T Qin Jun J Gu Wei W Li Dawei D Zhao Wenhui W

Nature communications 20200821 1

Oncogenic processes exert their greatest effect by targeting regulators of cell proliferation. Studying the mechanism underlying growth augmentation is expected to improve clinical therapies. The ovarian tumor (OTU) subfamily deubiquitinases have been implicated in the regulation of critical cell-signaling cascades, but most OTUs functions remain to be investigated. Through an unbiased RNAi screen, knockdown of OTUD5 is shown to significantly accelerate cell growth. Further investigation reveals ...[more]

PMID: 32826889

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