Proteomics

Dataset Information

0

Biomarkers of Cholangiocarcinoma and Pancreatic adenocarcinoma

ABSTRACT: Cholangiocarcinoma (CCA) and pancreatic adenocarcinoma (PDAC) may lead to the development of extrahepatic obstructive cholestasis. However, biliary stenoses can also be caused by benign conditions, and the identification of their etiology still remains a clinical challenge. We performed metabolomic and proteomic analyses of bile from patients with benign (n=36) and malignant conditions, CCA (n=36) or PDAC (n=57), undergoing endoscopic retrograde cholangiopancreatography with the aim of characterizing bile composition in biliopancreatic disease and identifying biomarkers for the differential diagnosis of biliary strictures. Comprehensive analyses of lipids, bile acids and small molecules were carried out using mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (1H-NMR) in all patients. MS analysis of bile proteome was performed in five patients per group. We implemented artificial intelligence tools for the selection of biomarkers and algorithms with predictive capacity. Our machine-learning pipeline included the generation of synthetic data with properties of real data, the selection of potential biomarkers (metabolites or proteins) and their analysis with neural networks (NN). Selected biomarkers were then validated with real data. We identified panels of lipids (n=10) and proteins (n=5) that when analyzed with NN algorithms discriminated between patients with and without cancer with an unprecedented accuracy.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bile

DISEASE(S): Pancreas Adenocarcinoma,Cholangiocarcinoma

SUBMITTER: Antonio Ramos Fernández  

LAB HEAD: Fernando J. Corrales

PROVIDER: PXD019924 | Pride | 2021-09-09

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Pilot Multi-Omic Analysis of Human Bile from Benign and Malignant Biliary Strictures: A Machine-Learning Approach.

Urman Jesús M JM   Herranz José M JM   Uriarte Iker I   Rullán María M   Oyón Daniel D   González Belén B   Fernandez-Urién Ignacio I   Carrascosa Juan J   Bolado Federico F   Zabalza Lucía L   Arechederra María M   Alvarez-Sola Gloria G   Colyn Leticia L   Latasa María U MU   Puchades-Carrasco Leonor L   Pineda-Lucena Antonio A   Iraburu María J MJ   Iruarrizaga-Lejarreta Marta M   Alonso Cristina C   Sangro Bruno B   Purroy Ana A   Gil Isabel I   Carmona Lorena L   Cubero Francisco Javier FJ   Martínez-Chantar María L ML   Banales Jesús M JM   Romero Marta R MR   Macias Rocio I R RIR   Monte Maria J MJ   Marín Jose J G JJG   Vila Juan J JJ   Corrales Fernando J FJ   Berasain Carmen C   Fernández-Barrena Maite G MG   Avila Matías A MA  

Cancers 20200621 6

Cholangiocarcinoma (CCA) and pancreatic adenocarcinoma (PDAC) may lead to the development of extrahepatic obstructive cholestasis. However, biliary stenoses can also be caused by benign conditions, and the identification of their etiology still remains a clinical challenge. We performed metabolomic and proteomic analyses of bile from patients with benign (<i>n</i> = 36) and malignant conditions, CCA (<i>n</i> = 36) or PDAC (<i>n</i> = 57), undergoing endoscopic retrograde cholangiopancreatograph  ...[more]

Similar Datasets

Proteomics New molecular mechanisms in cholangiocarcinoma: signals triggering interleukin-6 production in tumor cells and KRAS co-opted epigenetic mediators driving metabolic reprogramming.
2022-06-09 | PXD032802 | Pride
Proteomics Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype
2020-08-17 | PXD019289 | Pride
Genomics Serum exosome miRNA expression profile in patients with cholangiocarcinoma(CCA) and common bile duct stones(CBDS)
2022-10-20 | GSE215448 | GEO
Proteomics Identification and quantification of cholangiocarcinoma (CCA) marker proteins from bile extracellular vesicles for non-invasive diagnosis
2021-07-14 | PXD020349 | JPOST Repository
Proteomics Ectopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditions through proteome resetting
2023-02-15 | PXD036489 | Pride
Proteomics Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2
2018-12-11 | PXD010495 | Pride
Proteomics The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses
2020-08-11 | PXD017769 | Pride
Proteomics Serum and bile proteomics reveal candidates for new noninvasive serum markers of primary sclerosing cholangitis and of risk of disease progression and biliary neoplasia.
2022-08-03 | PXD026693 | Pride
Proteomics Serum and bile proteomics reveal candidates for new noninvasive serum markers of primary sclerosing cholangitis and of risk of disease progression and biliary neoplasia (BILE)
2022-08-03 | PXD026694 | Pride
Proteomics Persulfidated subproteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes and nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in plants.
2017-10-27 | PXD005168 | Pride