Dataset Information

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

ABSTRACT: Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo-β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β-D-glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Aspergillus Niger Nrrl3

SUBMITTER: Bogdan Florea

LAB HEAD: Bogdan I Florea

PROVIDER: PXD019930 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases.

de Boer Casper C McGregor Nicholas G S NGS Peterse Evert E Schröder Sybrin P SP Florea Bogdan I BI Jiang Jianbing J Reijngoud Jos J Ram Arthur F J AFJ van Wezel Gilles P GP van der Marel Gijsbert A GA Codée Jeroen D C JDC Overkleeft Herman S HS Davies Gideon J GJ

RSC chemical biology 20200728 3

Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, ...[more]

PMID: 34458755

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Dataset Information

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

Publications

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases.

Similar Datasets

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