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Targeted multiplexed proteomics of SARS-CoV-2 peptides in ALI cells

ABSTRACT: Human air-liquid-interface cultured cells (ALI) were infected with SARS-CoV-2 in presence or absence of the drug aprotinin. Targeted proteomics were performed on previously identified peptides modified with TMTpro.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: Kevin Klann

LAB HEAD: Christian Münch

PROVIDER: PXD019950 | Pride | 2020-11-05

REPOSITORIES: Pride

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Aprotinin Inhibits SARS-CoV-2 Replication.

Bojkova Denisa D Bechtel Marco M McLaughlin Katie-May KM McGreig Jake E JE Klann Kevin K Bellinghausen Carla C Rohde Gernot G Jonigk Danny D Braubach Peter P Ciesek Sandra S Münch Christian C Wass Mark N MN Michaelis Martin M Cinatl Jindrich J

Cells 20201030 11

Severe acute respiratory syndrome virus 2 (SARS-CoV-2) is the cause of the current coronavirus disease 19 (COVID-19) pandemic. Protease inhibitors are under consideration as virus entry inhibitors that prevent the cleavage of the coronavirus spike (S) protein by cellular proteases. Herein, we showed that the protease inhibitor aprotinin (but not the protease inhibitor SERPINA1/alpha-1 antitrypsin) inhibited SARS-CoV-2 replication in therapeutically achievable concentrations. An analysis of prote ...[more]

PMID: 33143316

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