Proteomics

Dataset Information

0

(p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains


ABSTRACT: Escherichia coli RelA is a ribosomal factor with strong (p)ppGpp synthesis activity that is dramatically activated in the presence of deacylated tRNA in the ribosomal A-site. RelA is a unique enzyme in that it is directly positively regulated by its product, alarmone nucleotide (p)ppGpp. Using HDX-MS, mulecular docking, biochemsitry, and microbiology approaches, we localise the (p)ppGpp binding site of E. coli RelA and uncover the molecular mechanism of RelA regulation by the alarmone.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Simon Ekström  

LAB HEAD: Vasili Hauryliuk

PROVIDER: PXD019953 | Pride | 2021-08-11

REPOSITORIES: Pride

Similar Datasets

2020-07-02 | PXD019367 | Pride
2013-08-30 | GSE50468 | GEO
2006-12-28 | GSE6618 | GEO
2013-08-30 | E-GEOD-50468 | biostudies-arrayexpress
2010-04-14 | E-MTAB-211 | biostudies-arrayexpress
2010-04-13 | E-MTAB-222 | biostudies-arrayexpress
2022-03-24 | PXD028663 | Pride
2020-03-09 | PXD015416 | Pride
2021-03-25 | PXD022185 | Pride
2019-11-12 | PXD013841 | Pride