Dataset Information

Anastrepha ludens antenna proteome in response to CeraTrap®

ABSTRACT: Anastrepha ludens (Loew) is one of the most important pestiferous fruit fly species infesting citrus and mangoes from Texas to Costa Rica. Despite the latter, the mechanisms of odorant perception in this specie have been poorly studied. In most of the cases, the detection of semiochemicals in insects is carried out in the antenna, where volatile molecules penetrate sensillum pores and they are linked to soluble proteins to cross the lymph until reaching specific odor receptor triggering the signal transduction which can lead to a behavioral response. Scrutinizing the molecular foundation of odorant perception in A. ludens is of paramount importance to increase the efficiency of Integrated Management strategies against this pest. Therefore, we carried out a proteomic profiling of A. ludens’s antennas with three different maturity stages using comparative proteomic analysis with tandem mass tags (TMT) combined with synchronous precursor selection (SPS)-MS3 to gain insight into the proteome of the A. ludens antenna under CeraTrap exposure, a highly effective commercial attractant. Our study provides the first report where critical molecular players might form part of the odor perception mechanism in A. ludens.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Anastrepha Ludens

TISSUE(S): Antenna

SUBMITTER: Eliel Ruiz-May

LAB HEAD: Laboratorio de Proteomica

PROVIDER: PXD020012 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant.

Ruiz-May Eliel E Altúzar-Molina Alma A Elizalde-Contreras José M JM Arellano-de Los Santos Jiovanny J Monribot-Villanueva Juan J Guillén Larissa L Vázquez-Rosas-Landa Mirna M Ibarra-Laclette Enrique E Ramírez-Vázquez Mónica M Ortega Rafael R Aluja Martín M

International journal of molecular sciences 20201029 21

Anastrepha ludens is a key pest of mangoes and citrus from Texas to Costa Rica but the mechanisms of odorant perception in this species are poorly understood. Detection of volatiles in insects occurs mainly in the antenna, where molecules penetrate sensillum pores and link to soluble proteins in the hemolymph until reaching specific odor receptors that trigger signal transduction and lead to behavioral responses. Scrutinizing the molecular foundation of odorant perception in A. ludens< ...[more]

PMID: 33138264

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Project description:Abscisic acid (ABA) is a key hormone for plant growth, development and stress adaptation. Perception of ABA through four types of receptors has been reported. We show here that impairment of ABA perception through the PYR/PYL/RCAR branch reduces vegetative growth and seed production, and leads to a severely open stomata and dramatic ABA insensitive phenotype, even though other branches for ABA perception remain functional. Arabidopsis sextuple mutant impaired in 6 PYR/PYL receptors, namely PYR1, PYL1, PYL2, PYL4, PYL5 and PYL8, was able to germinate and grow even on 100 mM ABA. Whole-rosette stomatal conductance (Gst) measurements revealed that leaf transpiration in the sextuple pyr/pyl mutant was higher than in the ABA-deficient aba3-1 or ABA-insensitive snrk2.6 mutants. The gradually increasing Gst values of plants lacking three, four, five and six PYR/PYLs indicate quantitative regulation of stomatal aperture by this family of receptors. The sextuple mutant lacked ABA-mediated activation of SnRK2s and ABA-responsive gene expression was dramatically impaired as was reported in snrk2.2/2.3/2.6. In summary, these results show that ABA perception by PYR/PYLs plays a major role to regulate seed germination and establishment, basal ABA signaling required for vegetative and reproductive growth, stomatal aperture and transcriptional response to the hormone. Four biological replicates were generated for the 3 genotypes, pyr1pyl1pyl2pyl4pyl5pyl8 sextuple mutant, snrk2.2/2.3/2.6 triple mutant, and Col-0. All the samples were treated with ABA for 3 hours before harvesting. The four Col-0 samples were mix to create an unique reference Col-0 sample. Two comparasion were made, fisrt one, PYR/PYL sextuple mutant versus Col-0 reference sample and second one, SnRK2 triple mutant versus Col-0 reference sample. In each comparasion four biological replicates were made. Replicas number 1 and 2 were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 reference sample, while the other two replicas,#3 and #4, were reversed-labeled.

Dataset Information

Anastrepha ludens antenna proteome in response to CeraTrap®

Publications

A First Glimpse of the Mexican Fruit Fly <i>Anastrepha ludens</i> (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant.

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