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Grad-Seq analysis of a photosynthetic bacterium rich in internal membrane systems

ABSTRACT: Here we analyzed the full ensemble of cellular RNAs and co-fractionating proteins using gradient profiling by sequencing (Grad-seq) in Synechocystis 6803, a cyanobacterium rich in internal membrane systems.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechocystis Sp. Pcc 6803

TISSUE(S): Cell Suspension Culture

SUBMITTER: Philipp Spaet

LAB HEAD: Wolfgang R Hess

PROVIDER: PXD020025 | Pride | 2022-02-15

REPOSITORIES: Pride

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Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq).

Riediger Matthias M Spät Philipp P Bilger Raphael R Voigt Karsten K Maček Boris B Hess Wolfgang R WR

The Plant cell 20210401 2

Although regulatory small RNAs have been reported in photosynthetic cyanobacteria, the lack of clear RNA chaperones involved in their regulation poses a conundrum. Here, we analyzed the full complement of cellular RNAs and proteins using gradient profiling by sequencing (Grad-seq) in Synechocystis 6803. Complexes with overlapping subunits such as the CpcG1-type versus the CpcL-type phycobilisomes or the PsaK1 versus PsaK2 photosystem I pre(complexes) could be distinguished, supporting the high q ...[more]

PMID: 33793824

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