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Targeted analysis of alternative protein isoforms in the human hearts

ABSTRACT: Human heart lysates acquired commercially were digested and processed with shotgun proteomics and parallel reaction monitoring with the aid of predicted spectra from Prosit.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart

SUBMITTER: Maggie Pui Yu Lam

LAB HEAD: Maggie PY Lam

PROVIDER: PXD020074 | Pride | 2022-05-19

REPOSITORIES: Pride

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Publications

Computation-assisted targeted proteomics of alternative splicing protein isoforms in the human heart.

Han Yu Y Wood Silas D SD Wright Julianna M JM Dostal Vishantie V Lau Edward E Lam Maggie P Y MPY

Journal of molecular and cellular cardiology 20210205

Alternative splicing is prevalent in the heart and implicated in many cardiovascular diseases, but not every alternative transcript is translated and detecting non-canonical isoforms at the protein level remains challenging. Here we show the use of a computation-assisted targeted proteomics workflow to detect protein alternative isoforms in the human heart. We build on a recent strategy to integrate deep RNA-seq and large-scale mass spectrometry data to identify candidate translated isoform pept ...[more]

PMID: 33549679

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