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Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression

ABSTRACT: Nuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we used an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins Nup153, Nup50 and Tpr. Here, we provide MS data for the Nuclear Pore-enriched fraction of human DLD-1 cells, expressing CRISPR-engeneered degron, fused with the endogenous locus of corresponding basket NUPs, in the absence or presence of Auxin.

INSTRUMENT(S): LTQ Orbitrap, Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Alexei Arnaoutov

LAB HEAD: Mary Dasso

PROVIDER: PXD020075 | Pride | 2020-06-30

REPOSITORIES: Pride

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Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway.

Aksenova Vasilisa V Smith Alexandra A Lee Hangnoh H Bhat Prasanna P Esnault Caroline C Chen Shane S Iben James J Kaufhold Ross R Yau Ka Chun KC Echeverria Carlos C Fontoura Beatriz B Arnaoutov Alexei A Dasso Mary M

Nature communications 20200911 1

Nuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we apply an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins NUP153, NUP50 and TPR. Acute depletion of TPR causes rapid and pronounce ...[more]

PMID: 32917881

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