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Proteomic analysis of successful and biologically compromised bone fracture healing

ABSTRACT: Bone fracture healing shows approximately 10-15% non-unions, although theoretically it has the potential of scarless regeneration. With regards to our aging society, a better understanding of the healing process is needed to allow for more sophisticated treatment options. Particular the early phase, which is a high complex and dynamic process in regards to nutrient and engery requirements. Here we investigate the proteomic (and metabolic) characteristics of the local microenvironment from successful (in young female Sprague-Dawley rats) and biologically compromised (in aged female rats with a minimum litter of three)bone reneration at day 3, 7 and 14 after osteotomy.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Bone, Bone Marrow

SUBMITTER: Julia Löffler

LAB HEAD: Stefan Kempa

PROVIDER: PXD020085 | Pride | 2023-05-10

REPOSITORIES: Pride

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A comprehensive molecular profiling approach reveals metabolic alterations that steer bone tissue regeneration.

Löffler Julia J Noom Anne A Ellinghaus Agnes A Dienelt Anke A Kempa Stefan S Duda Georg N GN

Communications biology 20230327 1

Bone regeneration after fracture is a complex process with high and dynamic energy demands. The impact of metabolism on bone healing progression and outcome, however, is so far understudied. Our comprehensive molecular profiling reveals that central metabolic pathways, such as glycolysis and the citric acid cycle, are differentially activated between rats with successful or compromised bone regeneration (young versus aged female Sprague-Dawley rats) early in the inflammatory phase of bone healin ...[more]

PMID: 36973478

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