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IRG1 Inflammasome Proteomics Experiments

ABSTRACT: BMDMs of indicated phenotypes were stimulated with LPS for 24h and analyzed or restimulated with LPS for additional 3h and analyzed. In some experiments 1mM itaconate was added at 4h of LPS first stimulation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Kate Lizotte

LAB HEAD: Sebastian Hayes

PROVIDER: PXD020089 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Itaconate confers tolerance to late NLRP3 inflammasome activation.

Bambouskova Monika M Potuckova Lucie L Paulenda Tomas T Kerndl Martina M Mogilenko Denis A DA Lizotte Kate K Swain Amanda A Hayes Sebastian S Sheldon Ryan D RD Kim Hyeryun H Kapadnis Unnati U Ellis Abigail E AE Isaguirre Christine C Burdess Samantha S Laha Anwesha A Amarasinghe Gaya K GK Chubukov Victor V Roddy Thomas P TP Diamond Michael S MS Jones Russell G RG Simons Donald M DM Artyomov Maxim N MN

Cell reports 20210301 10

Itaconate is a unique regulatory metabolite that is induced upon Toll-like receptor (TLR) stimulation in myeloid cells. Here, we demonstrate major inflammatory tolerance and cell death phenotypes associated with itaconate production in activated macrophages. We show that endogenous itaconate is a key regulator of the signal 2 of NLR family pyrin domain containing 3 (NLRP3) inflammasome activation after long lipopolysaccharide (LPS) priming, which establishes tolerance to late NLRP3 inflammasome ...[more]

PMID: 33691097

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Itaconate confers tolerance to late NLRP3 inflammasome activation

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