APOBEC3C, a perinucleolar protein induced by genotoxins, is recruited to DNA damage sites

ABSTRACT: The human genome contains 11 APOBEC (Apolipoprotein B mRNA Editing Catalytic Polypeptide-like) cytidine deaminases classified into four families. These proteins function mainly in innate antiviral immunity and can also restrict endogenous retrotransposable element multiplication. The present study focuses on APOBEC3C (A3C), a member of the APOBEC3 sub-family. Some APOBEC3 proteins use their enzymatic activity on genomic DNA, inducing mutations and DNA damage, while other members facilitate DNA repair. Our results show that A3C is highly expressed in cells treated with DNA damaging agents. Its expression is regulated by p53. In addition, we provide the A3C interactome in both normal conditions and in cells exposed to the genotoxin etoposide. Most A3C interacting partners are proteins involved in RNA metabolism and some in DNA repair. Our data also indicate that A3C overexpression is detrimental to mammalian cells and we show that this protein is located around the nucleolus and at laser-damaged DNA sites.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bone Tissue

DISEASE(S): Osteosarcoma

SUBMITTER: Patrice Waridel  

LAB HEAD: Christian Widmann

PROVIDER: PXD020105 | Pride | 2022-02-07

REPOSITORIES: Pride