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Naturally processed flucloxacillin haptenated HLA ligands

ABSTRACT: This study has demonstrated that natural flucloxacillin haptenated HLA-B*57:01 ligands arepresented by antigen presenting cells through multiple pathways. Identification of such epitopes will help to define the chemical signals responsible for drug-induced immunological disease.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Suspension Culture

DISEASE(S): Hypersensitivity Reaction Type Iv Disease,Drug Allergy

SUBMITTER: James Waddington

LAB HEAD: Xiaoli Meng

PROVIDER: PXD020137 | Pride | 2020-08-05

REPOSITORIES: Pride

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Identification of Flucloxacillin-Haptenated HLA-B*57:01 Ligands: Evidence of Antigen Processing and Presentation.

Waddington James C JC Meng Xiaoli X Illing Patricia T PT Tailor Arun A Adair Kareena K Whitaker Paul P Hamlett Jane J Jenkins Rosalind E RE Farrell John J Berry Neil N Purcell Anthony W AW Naisbitt Dean J DJ Park Brian Kevin BK

Toxicological sciences : an official journal of the Society of Toxicology 20201001 2

Flucloxacillin is a β-lactam antibiotic associated with a high incidence of drug-induced liver reactions. Although expression of human leukocyte antigen (HLA)-B*57:01 increases susceptibility, little is known of the pathological mechanisms involved in the induction of the clinical phenotype. Irreversible protein modification is suspected to drive the reaction through the modification of peptides that are presented by the risk allele. In this study, the binding of flucloxacillin to immune cells w ...[more]

PMID: 32726429

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