Proteomics

Dataset Information

0

Using BioID2 to map domain specific Ku70 protein interactors

ABSTRACT: To determine if the proximity-dependent biotin identification (BioID2) technique could identify and map domain and region specific protein interactors, we conducted BioID2 with various segments of the Ku70 protein in the HEK293 cell line and compared the different datasets. To our knowledge, this is the first study aimed at using BioID2 to directly identify domain and region specific protein interactors.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sanna Abbasi  

LAB HEAD: Caroline Schild-Poulter

PROVIDER: PXD020165 | Pride | 2021-04-22

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Identification of Ku70 Domain-Specific Interactors Using BioID2.

Abbasi Sanna S   Schild-Poulter Caroline C  

Cells 20210314 3

Since its inception, proximity-dependent biotin identification (BioID), an in vivo biochemical screening method to identify proximal protein interactors, has seen extensive developments. Improvements and variants of the original BioID technique are being reported regularly, each expanding upon the existing potential of the original technique. While this is advancing our capabilities to study protein interactions under different contexts, we have yet to explore the full potential of the existing  ...[more]

Similar Datasets

Proteomics Screening for Ku70 phosphoS155-dependent proximal proteins
2023-05-10 | PXD025581 | Pride
Unknown Identification of Ku70 Domain-Specific Interactors Using BioID2.
| S-EPMC8001828 | biostudies-literature
Transcriptomics Transcriptome of Yarrowia lipolytica strain E150 delta-Ku70 on glucose
2012-12-31 | E-MTAB-940 | biostudies-arrayexpress
Proteomics HEK293 Ku70 WT BioID - Mapping the Ku interactome using proximity-dependent biotin identification in human cells
2019-01-03 | PXD010931 | Pride
Proteomics Human Cardiac Fibroblast BioID2 Interactome for SKI and TAZ(WWTR1)
2021-09-09 | PXD018246 | Pride
Transcriptomics Ku80/Ku70 complex associated RNA in HEK293T cells
2024-04-09 | GSE219220 | GEO
Proteomics Generation and Characterization of a Conditional Ku70 Knockout Cell Line
2023-07-27 | PXD036297 | Pride
Genomics Ku70/HP1g are presented at the KCl induced acutely activated enhancers [ChIP-seq]
2022-08-04 | GSE185648 | GEO
Proteomics BioID2-Synapsin proteome Soderling
2021-07-15 | PXD019342 | Pride
Transcriptomics Ku70 Is a Novel Cytosolic DNA Sensor that Induces Type-III Rather than Type-I IFN
2011-01-29 | E-GEOD-26944 | biostudies-arrayexpress